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Related Articles簡要描述:Gibco™ MEM α, nucleosides, no ascorbic acid(Gibco MEM α培養(yǎng)基)is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.
Gibco MEM α培養(yǎng)基
Using MEM α:
MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system:
MEM α is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.