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Related Articles簡(jiǎn)要描述:Gibco™ BenchStable™ DMEM/F12 is a widely used basal medium for supporting the growth of many different mammalian cells.
Gibco™ BenchStable™ DMEM/F12 has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM/F12 when supplemented with ≥10% fetal bovine serum (FBS).
Using BenchStable DMEM/F-12:
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable DMEM/F-12 contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).
cGMP manufacturing and quality system:
Gibco BenchStable media is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.